Capybaras ( Hydrochoerus hydrochaeris) are also primary hosts of A. This tick species infests many species of both wild and domestic animals, although horses are the preferred hosts ( Labruna et al., 2001). cajennense Nava et al., 2014) and Amblyomma aureolatum, are incriminated as vectors ( Labruna, 2009).Īmblyomma sculptum is widely distributed in Brazil, mainly in the southeast region. In the Brazilian territory, Amblyomma sculptum (formely named A. rickettsii belong to the Amblyomma cajennense complex ( Labruna, 2009 Nava et al., 2014). In Central and South America, the most important species that transmit R. Amblyomma americanum and Rhipicephalus sanguineus are also implicated as vectors in the United States, respectively in the states of North Carolina and Arizona ( Demma et al., 2005 Breitschwerdt et al., 2011). rickettsii vectors, being Dermacentor variabilis and Dermacentor andersoni the main vectors in North America ( Dantas-Torres, 2007). Different tick species have been implicated as R. This disease is widely distributed in the Americas ( Dantas-Torres, 2007 Walker, 2007 Labruna, 2009), with high case fatality rates ( Angerami et al., 2006 Labruna, 2009). Rocky Mountain spotted fever (RMSF), caused by Rickettsia rickettsii, is the most lethal tick-borne rickettsiosis that affects humans ( Dantas-Torres, 2007). As result of their feeding on blood, these arthropods are versatile vectors of a wide variety of pathogenic microorganisms, such as viruses, bacteria, helminths, and protozoa ( Jongejan and Uilenberg, 2004 Dantas-Torres et al., 2012 Otranto et al., 2013). Ticks are obligate ectoparasites that infest numerous species of vertebrates. rickettsii transmission as well as pharmacological bioproducts with antihemostatic, anti-inflammatory and antibacterial activities. The functional characterization of the identified proteins might reveal potential targets to develop vaccines for tick control and/or blocking of R. To the best of our knowledge, this is the first report on the proteome of A. The corresponding CDSs of 59 of these proteins were upregulated in SG of fed ticks. Finally, high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis revealed 124 proteins in saliva of ticks fed for 96–120 h. Selected CDSs were analyzed by real-time quantitative polymerase chain reaction preceded by reverse transcription (RT-qPCR), corroborating the transcriptional profile obtained by RNA-seq. CDSs encoding secreted proteins, including lipocalins, mucins, protease inhibitors, glycine-rich proteins, metalloproteases, 8.9 kDa superfamily members, and immunity-related proteins were mostly upregulated by blood feeding. The total of reads obtained were assembled in 9,560 coding sequences (CDSs) distributed in different functional classes. sculptum ticks and those fed for 72 h on rabbits using next generation RNA sequencing (RNA-seq). In the current study, we compared the sialotranscriptome of unfed A. Besides facilitating tick feeding, the properties of saliva may also benefits survival and establishment of pathogens in the host. Tick saliva contains a complex mixture of bioactive molecules with anticlotting, antiplatelet aggregation, vasodilatory, anti-inflammatory, and immunomodulatory properties to counteract both the hemostasis and defense mechanisms of the host. Pathogens that are acquired with the blood meal must first colonize the tick gut and later the salivary glands (SG) in order to be transmitted during a subsequent blood feeding via saliva. It is known that the transmission of pathogens by ticks is mainly associated with the physiology of the feeding process. Amblyomma sculptum is vector of Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever (RMSF), the most lethal rickettsiosis that affects humans. Ticks are obligate blood feeding ectoparasites that transmit a wide variety of pathogenic microorganisms to their vertebrate hosts. 6Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Bethesda, MD, United States.5Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.4Departamento de Ciência da Computação, Instituto de Matemática e Estatística, Universidade de São Paulo, São Paulo, Brazil.3Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.2Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, Brazil.1Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.Maruyama 2 † Rebeca Kawahara 3 André Fujita 4 Larissa A.
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